Re: tc of VFT

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Date: Thu, 20 Nov 1997 05:59:10 -0500
From: Eric Cumbee <acumbee@surfsouth.com>
To: cp@opus.hpl.hp.com
Message-Id: <aabcdefg4435$foo@default>
Subject: Re: tc of VFT

Mr. Soler,
You might try using PPM. http://www.home.turbonet.com/ppm/ppmweb.htm A
procedure is listed there using PPM to clean explants before tissue
culturing.

Eric Cumbee
http://www.home.turbonet.com/ppm/ericnews.htm

Jessee G. Soler wrote:

> At 12:11 PM 11/19/97 -0800, you wrote:
> >Dear list,
> >
> >I recently tissue cultured some VFT petiole cuttings. I immersed the
> >cuttings in isopropyl alcohol for 3 minutes and then left them in
> >hydrogen peroxide for another 3 minutes. Today, after two days, I
> >checked up on the plants and found that they were turning an
> >unhealthy brown hue. I suspect that I might have 'over sterilized'
> >the plants. Does anyone know which substances are most effective in
> >sterilization?
> >
> >thanks,
> >
> >adwait kulkarni
> >
>
> A 10:1 water/bleach ..
> More later..
> J

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