Re: Tissue culture questions

Andreas Wistuba (A.Wistuba@DKFZ-Heidelberg.DE)
Sun, 9 Jan 1994 13:30:44 GMT+1

> Date: Fri, 7 Jan 1994 18:11:00 -0800
> Reply-to: <>
> From: Bob Cohen <>
> To: Multiple recipients of list <>
> Subject: Re: Tissue culture questions

> >
> >
> > Dear tissue culture gurus:
> >
> I took a stab at several genera using Carolina Biological Supply's VFT
> (dionea) multiplication media, which appears to be a weak MS type
> medium with a growth horomone added (I forget which one). I was
> particularly interested in Sarracenia, so I tried using explants from
> several locations - growth point, root tip, juvenile leaves, etc. No
> luck. The only genera I had any luck with was Pinguicula, which seems
> to propagate vegetatively at the drop of a hat anyway. I'm going to
> try using seeds in my next batch. Any tips appreciated.

I used meristems with one or two leaf primordia succesfully in case of
a few Sarracenia clones. It's a bit tricky working under the
stereo-microscope but I'd regard meristemming as absolutely
impossible without one.