Re: tissue culture

From: Chris Teichreb (
Date: Fri Jun 04 1999 - 12:59:38 PDT

Date: Fri, 4 Jun 1999 12:59:38 -0700 (PDT)
From: Chris Teichreb <>
Message-Id: <aabcdefg2004$foo@default>
Subject: Re: tissue culture


> hi all
> on the cambrian carnivores oline help and books i have read, pressure
> cookers are mentioned for the much needed sterilisation. what?,exactly is a
> pressure cooker?

        A sealed vessel which maintains a high temperature and pressure
while cooking. Often used for canning. They're just smaller versions of

        While regular boiling water will kill live bacterial and fungal
cells, it doesn't always kill the resting spores or cysts. The high
pressure disrupts the sturdy resting spore and prevents it from
germinating on your media.

> also, i am hoping to buy a tc kit sometime in the near
> future, how important is it to totaly sterilise everything used in tc, and
> are there any shortcuts for those of use who are unlucky enough not to be
> graced with the presence of a pressure cooker?

        You can try PPM (see Craig Gardner's web-site for details).
However, sterilization of all equipment, seed, plant material, is very
important. One fungal spore will very easily contaminate your entire
culture in short order. Some poeple have had success sterilizing
equipment and media in the microwave, although there's conflicting reports
about how effective this is.

> regards.
> chris.m.

Good luck!


Chris Teichreb
Department of Biological Sciences
Simon Fraser University
Burnaby, B.C.

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