Date: Tue, 9 Mar 1999 09:12:25 +0100 From: "Guy Van der Kinderen" <Guy.VanDerKinderen@rug.ac.be> To: cp@opus.hpl.hp.com Message-Id: <aabcdefg725$foo@default> Subject: Re: Byblis gigantea in vitro
Dear Brian,
Two years ago we (me and another member of vzw. Drosera) did manage
to transplant B. gigantea from vitro to normal substrate. The vitro
plants originated from Reginald Deroose, a commercial
vitro-cultivator, so I don't know anything about composition of
medium or treatment during cultivation. Of coarse, planlets were
hardened in the normal way, but no other special treatment was
applied. We transplanted three young plants which are still living
and growing at the moment. So, there might be another reason why your
plants always failed to grow out of vitro.
Kind regards,
Guy Van der Kinderen
vzw. Drosera, Belgium
> I saw a posting about starting Byblis gigantea in vitro and wanted
> to add this note that might be of interest.
>
> I tried for at least three years to get Byblis g. that were started
> in vitro out into normal soil and hardened off. At the time, I
> lived near Ron Gagliardo back when he ran Hungry Plants. He supplied
> me with numerous viles and flasks with which to experiment. I
> failed with all of them.
>
> Later, Ron told me that he really didn't know of anyone who had been
> successful in moving Byblis giganteas from the flasks to normal
> growing conditions. He suspected that the plants weren't forming a
> cuticle layer (I believe that's correct) which would allow them to
> harden in the air. This species is also extremely suseptible to
> fungus which probably didn't help matters. To this day, I don't know
> if anyone has been successful. I would be curious to hear.
>
> This is why, with Ron's and Allen Lowrie's help, I came upon a
> reliable way to start them from gibb-treated seed in milled
> sphagnum. It is the only way I recommend. Anyone starting them in
> vitro may want to hold back a few seeds just in case they have the
> same problems I did.
>
> Brian Cochran
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