Sterilization of explants. Help

From: Marin Martiskovich (
Date: Sun Mar 07 1999 - 22:05:22 PST

Date: Mon, 8 Mar 1999 01:05:22 -0500
From: "Marin Martiskovich" <>
Message-Id: <aabcdefg715$foo@default>
Subject: Sterilization of explants. Help

    Hello all. I am having problems with sterilization of explants from
Venus Fly Traps, that I am trying to tissue culture. From what I have read
on the subject most people seem to recommend first alcohol dipping for a
minute or two and then a 10% bleach solution for ten minutes. When I attempt
to do this I notice a purplish pigment leeching out of the explant much like
someone else experienced with seeds, except that I do not have any luck. The
explants look slightly limp, like steamed greens, but lighter in colour and
then they turn black on the medium within a day or so. Should I attempt to
reduce time in bleach solution or is 10 minutes at 10% basically essential
to sterilize plant material. Also has anyone attempted to use anything else
to sterilize explants with success? Possibly Dettol, triclosan, or any other
antibacterial compound.

This archive was generated by hypermail 2b30 : Tue Jan 02 2001 - 17:31:55 PST