Re: tissue culture requirements

Andreas Wistuba (A.Wistuba@DKFZ-Heidelberg.DE)
Thu, 19 Jan 1995 09:06:38 GMT+1

> Could one of the TC practitioners please describe what a laminar flow
> hood is (and why one prefers them to Volkswagens)?
A laminar flow hood is a compartment with a large (+/- one square
meter) filter through which air is blown. THe filter keeps particles
larger than 0,2 micrometers (the smallest known bacterial spores are
larger than that size). You sit opposed to the filter in a continuous
laminar stream of _sterile_ _dustfree_ air. BTW microchips, computer
hard drives and CD's are manufactured also in environments with ultra
clean air cleaned with the so called HEPA-filters.

They're better than Volkswagens because they produce cleaner air.
However I never tried to do tissue culture inside a Volkswagen.


Andreas Wistuba; Mudauer Ring 227; 68259 Mannheim; Germany
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Phone: +49-621-705471 Fax: +49-621-711307