Sterilization of VFT..

Jeffrey Michael Stein (
Wed, 18 Jan 1995 00:18:45 -0500 (EST)

> Also, tell me how you are sterilizing the tissue? I have grown many
> VFTs in vitro cultures initiated with seed and plant tissue. While
> tissue (ie. leaves, rhizome pieces, etc.) is hard to clean up, it
> can be done. Unopened flower buds are also valuable for this purpose.
> Ron Gagliardo Atlanta Botanical Garden
I've had great difficulty with sterilizing VFT tissue itself. Could you
possibly relay your proceedure, so one of us interestes can see. I've
tried a 2 min soak in 70% EtOH and then 5-10 minutes in 5% bleach. Alot
of tissue seems to die, and others are still contaminated. Strange.

Thank you.