Re: sowing seeds in vitro

Jan Schlauer (
Tue, 18 Jan 1994 16:45:39 +0100


>Which one of Sigma's agars do you use?

Sigma agar No. A-7002, Lot 57F-0170 (rather dirty stuff, but it works for me).

>Did you try this also with petri-dish cultures?

Yes, but they must be sealed carefully (tightly) with parafilm.

>I'm asking because I find petri dishes much more convenient and fast to handle
>>however I did not dare to sow seeds into dishes because dessication is much
>>quicker normally than in glass vials closed with foil and Parafilm.

Indeed, petri dishes are tricky for seeds (especially for the slow ones).
But you can "repot" seeds from nearly dried (i.e. NOT dry!) dishes to fresh
ones, and sometimes they germinate shortly after this step! I can only
state this for Lentibulariaceae, however (0 experience with other cp:
you'll have to try).

Kind regards