Re: N. bicalcarata seed

From: Edward Read (ay080@lafn.org)
Date: Mon Sep 18 2000 - 06:01:29 PDT


Date: Mon, 18 Sep 2000 06:01:29 -0700
From: "Edward Read" <ay080@lafn.org>
To: cp@opus.hpl.hp.com
Message-Id: <aabcdefg2960$foo@default>
Subject: Re: N. bicalcarata seed

Hello Stevan and all, Waiting for seeds to mature can be long and
    frustrating, but the rewards are worth it. I pollinated the
    flowers of the N. bicalcarata at California State Univerity at
    Fullerton. Both parents originated from R. Cantley many years
    ago. It took over two months for the seed to mature. The
    process may have been prolonged by my bagging the flowers with
    womens nylon (pantyhose) fabric before and after pollination.
    Literally thousands of seeds were produced, over half were
    donated. I observed a 90+% germination rate in vitro. Here is
    what I wrote in my notes: 9/20/99 - N. bicalcarata almost
    ready for pollination again. 12/10/99 - seed pods developing
    (every flower, even on those I did not bag and pollinate,
    therefore there are pollinators in the greenhouse, good thing I
    bagged the flowers well before they opened). 2/8/00 -pods still
    green, opened one to find seeds developed but nucleus is yellow
    and seed coat white. 3/11/00 - several pods opening. Nylon
    crowded, maybe I should change to a more open weaved fabric.
    Well, as you can read, I never recorded the days I pollinated
    the flowers. It was somewhere between 9/20/99 and 12/00/99. I
    kept the bags covered the entire time because I feared the seed
    falling to the floor while I was away on vacation.

Good Luck and have patience, Ed Read tissueculture@yahoo.com

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